Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Gold Biotechnology Inc Tris HCl 1 kg
Short for Tris (hydroxymethyl) aminomethane (THAM) hydrochloride, Tris HCl is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.
Alkali Scientific TRIS Buffered Saline, 10X Solution, 5 L
TRIS Buffered Saline (TBS) 10X solution is a versatile buffer solution used in molecular biology, biochemistry, and immunology. This 5L bottle is perfect for laboratories with high-volume requirements, providing an ample supply of concentrated buffer for a variety of assays and experiments. TBS is essential for maintaining a stable pH environment, which is crucial in protein assays, PCR, and cell culture applications. It is often used in antibody conjugation, cell washing, and sample dilution procedures, particularly in western blotting, ELISA, and other protein-based assays. This 10X solution can be easily diluted to a 1X working concentration, making it a flexible and reliable buffer for numerous laboratory applications.
![Alkali Scientific TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 4 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661279.jpg-250.jpg)
Alkali Scientific TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 4 L
TAE 10X Solution (TRIS Acetate EDTA), 4L, is a concentrated stock buffer solution that is essential for electrophoresis of nucleic acids. This 10X concentration is commonly used in molecular biology labs for gel preparation and other techniques that require a stable, low-ionic-strength buffer. The EDTA component chelates metal ions that could otherwise degrade nucleic acids, ensuring sample integrity during analysis. The larger 4L size is ideal for high-throughput laboratories that require large volumes of buffer for routine electrophoresis, making it a cost-effective solution for larger experiments or for labs processing multiple samples simultaneously.
Gold Biotechnology Inc Bis-Tris 1 kg
Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer that is useful for a pH range of 5.8 – 7.2. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.
New England Biolabs, Inc. Monarch Buffer WX - 40 ml
The Monarch Buffer WX, a component of Monarch Nucleic Acid Purification Kits, is designed for wash steps in nucleic acid purification. It is supplied as a 5X concentrate and requires dilution with ethanol (>=95%) before use. The product is supplied in 2 bottles, each with 20 ml of concentrated buffer.
- A component of Monarch Nucleic Acid Purification Kits
- Designed for wash steps in nucleic acid purification
- Monarch kit components are also available separately for added convenience
![Research Products International Corp RPI TBE 10X Solution [TRIS-borate-EDTA 10X Solution], 10 Liters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T32020-10000.0.JPG-250.jpg)
Research Products International Corp RPI TBE 10X Solution [TRIS-borate-EDTA 10X Solution], 10 Liters
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.2 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.2 (0.2 micron filtered), 500 ml.
Teknova 1M Tris-HCl, pH 9.0 Solution, 1000mL, Sterile. 2 Pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Sigma Aldrich Fine Chemicals Biosciences Tris Acetate-EDTA Buffer 51L
TRIS Tris Acetate-EDTA Buffer 51L
Selleck Chemical LLC Trometamol S4176-50mg
Trometamol(Tromethamine) is a proton acceptor used to treat acidemia